Anti-diabetic study and its Quantitative Real-time PCR analysis of the INSR gene in

Abstract:

Manufacturing metal oxide nanoparticles for chemical and biomedical applications is made possible by green synthesis, an eco-friendly approach.This investigation examined how silver nanoparticle leaf extract affected gene expression and its ability to treat diabetes.In this study, the Anti-diabetic study was evaluated by following the Glucose utilization control method. Furthermore, the study continues with Quantitative Real Time-PCR Analysis of the INSR Gene.The analysis’s findings showed that, when used as a control, metformin exhibits a glucose percentage of 169.26, whereas silver nanoparticle extracts exhibit a percentage

control of 169.53.HepG2 cells treated with AgNPsC.cuspidtus showed upregulated INSR gene expression, going from 1-fold in control cells to 1.51-fold in treated cells. The relative gene expression of the control is typically set to 1 when compared to the sample.The study shows that glucose absorption in AgNPs extract is slightly higher than in methanolic extract.As a result, there is a pressing mandate to protect this species and investigate its biological applications to achieve more significant and worthwhile outcomes.

Keywords: INSR genes, RT-PCR, Anti-diabetic, Silver nanoparticles, Hep G2 Cells