Green Synthesis of Silver Nanoparticle by Ziziphus mauritiana Leaf extract and its antifungal activity against Aspergillus species

Abstract:

In recent years, the use of biological agents, such as plant extracts, for the green synthesis of nanoparticles (NPs) has become increasingly popular. This study focuses on the bio synthesis of silver NPswith the help of leaf extract from Ziziphus mauritianaalog with its potential antifungal action against plant pathogenic fungi. The study investigated several parameters, including AgCl2 concentration (ranging from 1 to 100 mM), aqueous extract (100 to 900 µL), pH (4 to 10), incubation time (15 to 120 min), and temperature (30°C, 60°C, and 90°C) to determine their effect on the synthesis of AgNPs. The optimal conditions for synthesis were found to be a 100 mM concentration of AgCl2, a temperature of 30 °C, a pH of 6.0, and a duration of 120 min. The antifungal activity of the synthesized nanoparticles was evaluated against Aspergillus terreus and Aspergillus niger, and it was found to exhibit 53.5% and 65.1% inhibition rate, respectively, as measured by colony formation assay. The NPs were characterized by UV-Visible spectroscopy, which showed an absorption peak at around 460 nm. X-ray diffraction (XRD) revealed that the synthesized nanoparticles showed a crestline structure with a size extending from 10 to 100 nm, and SEM analysis demonstrated that they were nearly spherical in shape with no agglomeration. Furthermore, the antifungal study demonstrated that these silver NPs had potent antifungal action.